Fig. 3.

Knocking down Zeb2-NAT affects Zeb2 expression. Fibroblasts from adult mice were transfected with either control LNA Gapmers or oligonucleotides targeting Zeb2-NAT and Zeb2. a qRT-PCR analysis of Zeb2-NAT RNA. b qRT-PCR analysis of total Zeb2 transcripts (primer Zeb2 #1, see Supplementary Table 2). c Immunoblot for Zeb2 and β-actin in total cell lysates after transfection of anti-Zeb2-NAT Gapmers #1 and #2 (see Supplementary Fig. 2b). d qRT-PCR analysis of spliced and unspliced Zeb2 RNA. Double arrows indicate the position of primers. For all graphics depicted, Student’s t test (two-tailed) statistics, *p < 0.05, **p < 0.01, ***p < 0.001; error bars represent standard deviation; n=experiments with independent cell cultures