Fig. 7.

Blocking expression of Zeb2-NAT in ES cells enhances pluripotency and self-renewal. a qRT-PCR analysis of Nanog RNA in TNG-A cells transfected with the indicated oligonucleotides and grown in the presence (+) or absence (−) of 2i for 48 h. Transcript levels were normalized to GAPDH mRNA and depicted as fold change. Student’s t test (two-tailed) statistics, *p < 0.05, ***p < 0.001; error bars represent standard deviation. At least three independent experiments were carried per condition. b Immunoblot for Nanog and β-actin in total cell lysates from TNG-A cells transfected as indicated and grown in the presence (+) or absence (-) of 2i for 48 h. Relative quantification was carried out using Scion Image Software. c qRT-PCR analysis of E-cadherin RNA in TNG-A cells transfected as indicated and grown in the presence (+) or absence (−) of 2i for 48 h. Transcript levels were normalized to GAPDH mRNA and depicted as fold change. Student’s t test (two-tailed) statistics, *p < 0.05; error bars represent standard deviation. At least three independent experiments were carried per condition. d Histological section of a subcutaneous teratoma containing mesoderm (mes), endoderm (end), and ectoderm (ect), at 7 weeks after injection of cells treated with anti-Zeb2-NAT oligonucleotides. e Quantification of tumor volume (mm³). Student’s t test (two-tailed) statistics, *p < 0.05; error bars represent standard deviation; n=teratomas from independent cell cultures. f Representative images of tumors observed at 7 weeks after cell transplantation. Magnifications as indicated