Table 2.
List of synthetic DNA and RNA oligos used in the study.
| miRNA mimics, designed as dsRNA oligonucleotides r- RNA bases; r_ * - Phosphorothioated RNA base; m- 2’ O-methyl RNA base | ||
|---|---|---|
| Anti- sense mimic | Sense mimic | |
| miR-142-3p | rU*rG*rUrArGrUrGrUrUrUrCrCrUrArCrUrUrUrArUmGmGrA | 5SpC3/rUmCrCmArUmArAmArGmUrAmGrGmArAmArCmArCmUrAmCA |
| miR-9-5p | rU*rC*rUrUrUrGrGrUrUrArUrCrUrArGrCrUrGrUrAmUmGrA | mAmUrAmCrAmGrCmUrAmGrAmUrAmArCmCrAmArAmGA |
| miR-10a-5p | rU*rA*rCrCrCrUrGrUrArGrArUrCrCrGrArArUrUrUmGmUrG | mCmArAmArUmUrCmGrGmArUmCrUmArCmArGmGrGmUA |
| miR-29a-3p | rU*rA*rGrCrArCrCrArUrCrUrGrArArArUrCrGrGmUmUrA | mAmCrCmGrAmUrUmUrCmArGmArUmGrGmUrGmCrUA |
| miR-30c-5p | rU*rG*rUrArArArCrArUrCrCrUrArCrArCrUrCrUrCmAmGrC | mUmGrAmGrAmGrUmGrUmArGmGrAmUrGmUrUmUrAmCA |
| miR-124-3p | rU*rA*rArGrGrCrArCrGrCrGrGrUrGrArArUmGmCrC | mCmArUmUrCmArCmCrGmCrGmUrGmCrCmUrUA |
| miR-135a-5p | rU*rA*rUrGrGrCrUrUrUrUrUrArUrUrCrCrUrArUrGmUmGrA | mAmCrAmUrAmGrGmArAmUrAmArAmArAmGrCmCrAmUA |
| miR-138-5p | rA*rG*rCrUrGrGrUrGrUrUrGrUrGrArArUrCrArGrGmCmCrG | mGmCrCmUrGmArUmUrCmArCmArAmCrAmCrCmArGmCU |
| miR-206-3p | rU*rG*rGrArArUrGrUrArArGrGrArArGrUrGrUrGmUmGrG | mAmCrAmCrAmCrUmUrCmCrUmUrAmCrAmUrUmCrCA |
| miR-218-5p | rU*rU*rGrUrGrCrUrUrGrArUrCrUrArArCrCrAmUmGrU | mAmUrGmGrUmUrAmGrAmUrCmArAmGrCmArCmAA |
| Control | rC*rG*rCrGrArCrUrArUrArCrGrCrGrCrArArUrArUmGmGrU | rAmCrCmArUmArUmUrGmCrGmCrGmUrAmUrAmGrUmCrGmCG |
| Quantitative real time PCR DNA primers | ||
| Gene | Forward | Reverse |
| Cfl2 | TCTCGTCCCAGTGCCACCGA | ACTCCAGATGCCATAGTGCCCGC |
| Hprt | CTGGTTAAGCAGTACAGCCCCAAA | TGGCCTGTATCCAACACTTCGAGA |
| Vim | AAATGGCTCGTCACCTTCGT | AGAAATCCTGCTCTCCTCGC |
| Ptbp1 | ATTCCGTGTGGTCACAGACA | GTCACTGGAAGGAGCTCAGG |
| Mdk | CCAGGAGACCATCCGCG | TCCTTTTCCTTTCTTGGCTTTG |
| miR-142-3p | TGTAGTGTTTCCTACTTTATGGA | Universal primer (Qiagen) |
| miR-9-5p | TCTTTGGTTATCTAGCTGTATGA | Universal primer (Qiagen) |
| miR-10a-5p | TACCCTGTAGATCCGAATTTGTG | Universal primer (Qiagen) |
| miR-29a-3p | TAGCACCATCTGAAATCGGTTA | Universal primer (Qiagen) |
| miR-30c-5p | TGTAAACATCCTACACTCTCAGC | Universal primer (Qiagen) |
| miR-124-3p | TAAGGCACGCGGTGAATGCC | Universal primer (Qiagen) |
| miR-135a-5p | TATGGCTTTTATTCCTATGTGA | Universal primer (Qiagen) |
| miR-138-5p | AGCTGGTGTTGTGAATCAGGCCG | Universal primer (Qiagen) |
| miR-206-3p | TGGAATGTAAGGAAGTGTGTGG | Universal primer (Qiagen) |
| miR-218-5p | TTGTGCTTGATCTAACCATGT | Universal primer (Qiagen) |
| RNU6B (U6) | GATGACACGCAAATTCGTGAA | Universal primer (Qiagen) |