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. 2018 Jan 8;8:119. doi: 10.1038/s41598-017-18375-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aging impacts the Wnt responsiveness and osteogenic potential of BGM. (A) Bone grafts were harvested from young and aged mice and the marrow component was isolated then cultured to separate non-adherent from the adherent cell population containing stromal/stem cells. The total number of cells in each fraction was calculated⁴⁷. In these cultured cell populations (B) immunostaining for Stro-1, CD105, and CD73 was performed. (C,D) Micro-CT imaging of the skeletons of young and (E,F) aged mice. Color mapping of cortical and trabecular thicknesses, obtained from µCT data, are shown from representative (G) young and (H) aged mice. (I) Quantitative RT-PCR expression of Axin2 and Osterix in the mineralized matrix components of bone grafts harvested from young (light blue bars) and aged (dark blue bars) mice. Xgal staining of the marrow from (J) young and (K) aged Axin2 ^LacZ/+ mice. Scale bars = 50 µm.