Fig. 5.

TCC deletion in IFNAR1 decreases type I IFN signaling in human cells. HEK 293T cells were transfected with Cas9-expressing vector with or without endogenous IFNAR1 targeting gRNA. The endogenous IFNAR1-silenced (gRNA+) or control (gRNA−) cells were then transfected with WT or mutant IFNAR1 expression vectors together with an ISRE-driven luciferase reporter plasmid. a The luciferase activity was analyzed 18 h after IFN-α or IFN-β stimulation. The data shown are mean ± S.D. relative luciferase intensity units of triplicate cultures. b The expression of ISGs in WT (black) or TCC-deleted IFNAR1 (red) transfected IFNAR1 knockout HEK293 cells at 6 h after stimulation with IFN-β determined using qRT-PCR. The data shown are mean fold increase ± S.D. (triplicate cultures) over unstimulated controls. Data shown are representative of 2–3 independent experiments