Fig. 6.

TCC deletion in IFNAR1 decreases the affinity of IFN-β for IFNAR1. a Model of human IFNAR1 (brown; SD1–4) in complex with IFN-β (blue) and the location of Pro335 deletion. The model was generated using the crystal structure of the homologous mouse IFNAR1/IFN-β complex (PDB code 3WCY⁵³), illustrating overall receptor structure and position of the identified deleted residue (P335; green) in the inter-domain hinge between SD3 and SD4 of human IFNAR1. b, c The effect of TCC deletion on the IFNAR1 and IFN-β interaction. The affinity of human IFN-β for recombinant forms of WT IFNAR1-ECD (black) or IFNAR1-ECD-Del (red) was measured using SPR. b Sensorgram showing the kinetics of cytokine–cytokine receptor interaction. The mean response units (RU) measured over the course of triplicate experiments are shown over time. The vertical dotted line in the sensorgram represents the transition from the association phase (the “on” phase from 0–60 s) to the dissociation phase (the “off” phase from 61 s to the end). c Summary data showing the mean response units ± S.D. of three independent experiments. *p < 0.05 (Students t-test)