Figure 4. Cell proliferation in crypts in mucosal biopsies from healthy subjects treated with linaclotide or placebo in Stage I.
Proliferation was quantified by enumerating cells expressing Ki67 by immunofluorescence. Ki67 was enumerated in 10–20 crypts in each biopsy and means were calculated. For each intestinal segment, the ratio of mean Ki67 expression on day 1 (pre-dose) and 7 (post-dose) were calculated. C, cecum; TC, transverse colon, R, rectum. ***, p<0.001.