Table 2. Quantification of the Malat-1 ISH-signal in three different cell lines using four different probe technologies.
| HeLa | NHDF | T47D | ||
|---|---|---|---|---|
| Stellaris | MALAT1 nuc | 39 ± 2 N = 129 | 50 ± 2 N = 114 | 93 ± 23 N = 211 |
| MALAT1 cyt | 20 ± 1 N = 129 | 22 ± 2 N = 114 | 34 ± 1 N = 211 | |
| nuc-cyt | 19 | 28 | 59 | |
| nuc/cyt ratio | 1.9 | 2.3 | 2.7 | |
| ViewRNA | MALAT1 nuc | 186 ± 12 N = 132 | 18 ± 2 N = 117 | 78 ± 5 N = 227 |
| MALAT1 cyt | 24 ± 4 N = 132 | 8 ± 1 N = 117 | 28 ± 2 N = 227 | |
| nuc-cyt | 162 | 10 | 50 | |
| nuc/cyt ratio | 7.6 | 2.3 | 2.8 | |
| LNA+TSA | MALAT1 nuc | 456 ± 19 N = 139 | 246 ± 10 N = 123 | 774 ± 27 N = 215 |
| MALAT1 cyt | 150 ± 7 N = 139 | 63 ± 3 N = 123 | 299 ± 9 N = 215 | |
| nuc-cyt | 307 | 183 | 475 | |
| nuc/cyt ratio | 3.1 | 3.9 | 2.6 | |
| Stellaris+TSA | MALAT1 nuc | 693 ± 40 N = 91 | 357 ± 23 N = 88 | 991 ± 34 N = 149 |
| MALAT1 cyt | 292 ± 22 N = 91 | 91 ± 6 N = 88 | 393 ± 12 N = 149 | |
| nuc-cyt | 401 | 266 | 598 | |
| nuc/cyt ratio | 2.4 | 3.9 | 2.5 |
The mean signal intensity ± the standard error of mean is given based on the quantification of individual cells (N). In each cell, the mean pixel intensity has been determined in the nucleus (nuc) and the cytoplasm (cyt), respectively. The nuc-cyt value has been calculated by subtracting the signal of the cytoplasm from the signal of nucleus. In addition, the ratio of the signal in the nucleus and the cytoplasm (nuc/cyt ratio) has been calculated.