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. 2017 Oct 23;46(1):e3. doi: 10.1093/nar/gkx940

Figure 3.

Figure 3.

Assessing the quality and quantity of RNA isolated with linear or optimized non-linear gradients. (A) Mean (error bars correspond to standard deviations) amount of RNA (log2 ng) from quadruplicate experiments using linear or optimized non-linear gradients for both HCT-116 p53+/+ and HCT-116 p53−/− cells (the cytosolic lysate obtained from three 15 cm plates of 80% confluent cells was applied to each gradient). (B) Obtained RNA integrity numbers (RIN) for samples processed using linear and optimized non-linear gradients (same experiment as A).