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. 2017 Nov 23;46(1):456–472. doi: 10.1093/nar/gkx1164

Figure 5.

Figure 5.

Effect of RcsB mutations in capsule formation and motility. Overexpression of RcsB mutants in the Salmonella Typhimurium strain MD4821 (igaA1 rcsB) to monitor effect on colanic capsule production and motility in vivo. (A) Expression of the WT, unphosphorylatable mutant D56A and empty vector (as control). (B) Expression of mutants at residues present in the DBD domain that either interact with DNA according to the model RcsBBeF–DNA or contribute to DBD–DBD dimerization (L202). (C) Expression of mutants at residues that contribute to specific interactions between REC and DBD at each subunit. (D) Expression of mutants at relevant residues H12 and M88 that contribute to RcsB dimerization and catalysis. (E) Expression of mutants at residue S207C that traps DBD–DBD in the crossed conformation due to disulfide bond formation. A double mutant (S207C/M88A) containing an additional mutation M88A is also shown.