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. 2017 Nov 9;46(1):387–402. doi: 10.1093/nar/gkx1083

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Subunit composition of subassemblies of the RNA degradosome. The left panels show cartoon schematics of the subassemblies (ternary and binary complexes); the central panels show analysis by SEC-MALS in which LS (red) and differential refractive index (dRI, blue) are plotted in addition to M_w (gray); the right panels present SDS-PAGE images for the peak fractions. (A) The portion of RNase E encompassing residues 603–850 (blue) (with C-terminal Hexa-histidine tag; hereafter RNase E 603–850) interacts with RhlB (orange) and enolase (pink) to form a stable ternary complex composed RNase E 603–850: enolase: RhlB in a 1:2:1 molar ratio as indicated by the predicted and observed M_w. (B) RNase E 603–850 and RhlB form a stable binary assembly of 1:1 RNase E 603–850: RhlB. Predicted and observed M_w of the ternary and binary complexes, with their polydispersity values are shown in the table (inset). Note: RNase E 603–850 migrates slightly slower than expected, possibly due to highly charged regions or high proline content within the peptide (14,56).