Figure 3.

Mutagenic end-joining is suppressed in the presence of DNA-PKcs inhibitor upon depletion of Pol β. Quantification of the flow cytometry plots for U2OS EJ-DRs cells. Cells were treated with a non-targeting siRNA (C) or siRNA 1 that targets the POLB gene (si1). NU7741 is an inhibitor of DNA-PKcs and its addition to the cells is indicated by +. The addition of the ligands needed to induce expression of I-SceI is indicated by +. The percentage of RFP+ cells plotted on the Y-axis. Data are graphed as mean ± SEM (n = 3) ** (P ≤ 0.004), * (P = 0.02) and P = ns (not significant).