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. 2017 Nov 17;46(1):242–255. doi: 10.1093/nar/gkx1147

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Pol β-depleted cells have increased levels of DSBs upon treatment with BLM or NU7441. (A) MCF7 and MCF7-sh-POLB cells were treated with 50 μg/ml BLM for 1 h and allowed to recover for 0, 2 and 4 h. DSBs were analyzed by the neutral comet assay. The tail moment is plotted on the Y-axis. ** (P = 0.0028); ****(P < 0.0001). (B) U2OS and U2OS-sh-POLB cells were treated with 50 μg/ml BLM for 1 h and allowed to recover for 0, 2 and 4 h. DSBs were analyzed by the neutral comet assay. The tail moment is plotted on the Y-axis. * (P = 0.03); ** (P = 0.005); ****(P < 0.0001). (C) Representative images from each time point of recovery post BLM treatment for U2OS and U2OS Pol β-depleted cells. (D) MCF7 and MCF7-sh-POLB cells were treated with 10 μM NU7441 for 24 h. DSBs were analyzed by the neutral comet assay. The tail moment is plotted on the Y-axis. ** (P = 0.0012). (E) WT U2OS and U2OS-sh-POLB cells were treated with 10 μM NU7441 for 24 h. DSBs were analyzed by the neutral comet assay. The percentage of tail DNA is plotted on the Y-axis. * (P = 0.020). (F) MCF7 and MCF7-sh-POLB cells were treated with 10 μM NU7441 for 24 h and then treated with 50 μg/ml BLM for 1 h and allowed to recover for 0, 2 and 4 h. DSBs were analyzed by the neutral comet assay. The tail moment is plotted on the Y-axis. ** (P = 0.003); *** (P = 0.0006); ****(P < 0.0001). (G) U2OS and U2OS-sh-POLB cells were treated with 10 μM NU7441 for 24 h and then treated with 50 μg/ml BLM for 1 h and allowed to recover for 0, 2 and 4 h. DSBs were analyzed by the neutral comet assay. The tail moment is plotted on the Y-axis. * (P = 0.03); ** (P = 0.009); ****(P < 0.0001).