Figure 5.

ER-RNA expression in transgenic mouse models of DM1. (A) cDNA slot blot of total cellular RNA from HSA^LR quadriceps (Quads, 2 μg, three different mice) using Superscript-III (SS-III) or TGIRT-III. (B) cDNA slot blot of 2 μg total cellular RNA from quadriceps or tibialis anterior (TA) muscles from HSA^LR and HSA^XLR mice. Bottom-most wells in each column are from HSA^SR or HSA^NR mice, which do not express ER-RNA. (C) Relative amount of ER-RNA by cDNA slot blot in HSA^LR and HSA^XLR quadriceps and TA muscle (black bars, mean signal in HSA^LR quadriceps set to 1), as compared to inferred ER-RNA level based on qRT-PCR (white bars, calculated by multiplying expression level x repeat length, with the mean product in HSA^LR quadriceps set to 1). Results are based on n = 3 for HSA^LR and HSA^XLR mice. HSA transgene expression by qRT-PCR was normalized to general transcription factor 2b (Gtf2b).