Table 1. Primer and probe sequences.
Name | Type | Sequence (5′→3′) |
---|---|---|
pDWD-specific *, ‡ | DNA RT primer | CGGGTGGGTTACCGAAGAC |
pDWD-specific hybrid | rna/DNA RT primer | cgggtgggttaccgaAGAC |
(CAG)6+1 | DNA RT primer | CAGCAGCAGCAGCAGCAGC |
(CAG)6+1 hybrid | rna/DNA RT primer | cagcagcagcagcagCAGC |
(CAGG)4+2 * | DNA RT primer | CAGGCAGGCAGGCAGGCA |
(CAGG)4+2 hybrid | rna/DNA RT primer | caggcaggcaggcaGGCA |
(GGCCCC)3+3 * | DNA RT primer | GGCCCCGGCCCCGGCCCCGGC |
(GGCCCC)3+3 hybrid | rna/DNA RT primer | ggccccggccccggcccCGGC |
(GGGGCC)3+3 * | DNA RT primer | CGGGGCCGGGGCCGGGGCCGG |
(GGGGCC)3+3 hybrid | rna/DNA RT primer | cggggccggggccggggCCGG |
(CTG)7 LNA Δ | Digoxigenin LNA Probe | CTGCTGCTGCTGCTGCTGCTG |
(CCTG)6 LNA Δ | Digoxigenin LNA Probe | CCTGCCTGCCTGCCTGCCTGC |
(GGGGCC) LNA Δ | Digoxigenin LNA Probe | GGGGCCGGGGCCGGGGCC |
hACTA1-Ex1F | qPCR forward primer | CCAGGCCGGAGCCATT |
hACTA1-Ex2R | qPCR reverse primer | GTAGCTACCCCGCCCAGAAACT |
hACTA1-probe | qPCR probe | ACCACCGCCCTCGTGTGCG |
hDMPK 3′UTR-F | qPCR forward primer | CTATCGTTGGTTCGCAAAGTG |
hDMPK 3′UTR-R | qPCR reverse primer | GCAAATTTCCCGAGTAAGCAG |
hDMPK 3′UTR-probe | qPCR probe | AAGCTTTCTTGTGCATGACGCCC |
mSerca1 Ex22-F † | RT-PCR splicing primer | CTCATGGTCCTCAAGATCTCAC |
mSerca1 Ex22-R | RT-PCR splicing primer | GGGTCAGTGCCTCAGCTTTG |
mClcn1 Ex7A-F † | RT-PCR splicing primer | TGAAGGAATACCTCACACTCAAGG |
mClcn1 Ex7A-R | RT-PCR splicing primer | CACGGAACACAAAGGCACTG |
mTmem63b Ex5-F † | RT-PCR splicing primer | CTGGCTCTGGACTTCATGTGCTTTC |
mTmem63b Ex5-R | RT-PCR splicing primer | GAGACGGAGGTGAGACGCTCATACC |
mCacna1s Ex29-F † | RT-PCR splicing primer | GAGATCCTTGGAATGTGTTTGACTTCCT |
mCacna1s Ex29-R | RT-PCR splicing primer | GGTTCAGCAGCTTGACCAGTCTCAT |
Key: N = DNA, n = RNA, N = LNA. RT primers 5′-end-labeled with HEX (*), FAM (†), IRD-700 (‡), or Digoxigenin (Δ).