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. 2017 Oct 3;46(1):e1. doi: 10.1093/nar/gkx867

Table 1. Primer and probe sequences.

Name Type Sequence (5′→3′)
pDWD-specific *, ‡ DNA RT primer CGGGTGGGTTACCGAAGAC
pDWD-specific hybrid rna/DNA RT primer cgggtgggttaccgaAGAC
(CAG)6+1 DNA RT primer CAGCAGCAGCAGCAGCAGC
(CAG)6+1 hybrid rna/DNA RT primer cagcagcagcagcagCAGC
(CAGG)4+2 * DNA RT primer CAGGCAGGCAGGCAGGCA
(CAGG)4+2 hybrid rna/DNA RT primer caggcaggcaggcaGGCA
(GGCCCC)3+3 * DNA RT primer GGCCCCGGCCCCGGCCCCGGC
(GGCCCC)3+3 hybrid rna/DNA RT primer ggccccggccccggcccCGGC
(GGGGCC)3+3 * DNA RT primer CGGGGCCGGGGCCGGGGCCGG
(GGGGCC)3+3 hybrid rna/DNA RT primer cggggccggggccggggCCGG
(CTG)7 LNA Δ Digoxigenin LNA Probe CTGCTGCTGCTGCTGCTGCTG
(CCTG)6 LNA Δ Digoxigenin LNA Probe CCTGCCTGCCTGCCTGCCTGC
(GGGGCC) LNA Δ Digoxigenin LNA Probe GGGGCCGGGGCCGGGGCC
hACTA1-Ex1F qPCR forward primer CCAGGCCGGAGCCATT
hACTA1-Ex2R qPCR reverse primer GTAGCTACCCCGCCCAGAAACT
hACTA1-probe qPCR probe ACCACCGCCCTCGTGTGCG
hDMPK 3′UTR-F qPCR forward primer CTATCGTTGGTTCGCAAAGTG
hDMPK 3′UTR-R qPCR reverse primer GCAAATTTCCCGAGTAAGCAG
hDMPK 3′UTR-probe qPCR probe AAGCTTTCTTGTGCATGACGCCC
mSerca1 Ex22-F † RT-PCR splicing primer CTCATGGTCCTCAAGATCTCAC
mSerca1 Ex22-R RT-PCR splicing primer GGGTCAGTGCCTCAGCTTTG
mClcn1 Ex7A-F † RT-PCR splicing primer TGAAGGAATACCTCACACTCAAGG
mClcn1 Ex7A-R RT-PCR splicing primer CACGGAACACAAAGGCACTG
mTmem63b Ex5-F † RT-PCR splicing primer CTGGCTCTGGACTTCATGTGCTTTC
mTmem63b Ex5-R RT-PCR splicing primer GAGACGGAGGTGAGACGCTCATACC
mCacna1s Ex29-F † RT-PCR splicing primer GAGATCCTTGGAATGTGTTTGACTTCCT
mCacna1s Ex29-R RT-PCR splicing primer GGTTCAGCAGCTTGACCAGTCTCAT

Key: N = DNA, n = RNA, N = LNA. RT primers 5′-end-labeled with HEX (*), FAM (†), IRD-700 (‡), or Digoxigenin (Δ).