Figure 1.

Snail1 loss causes telomere dysfunctions. (A) Expression of Snail1 at late passages (PD 146) of control MSC (CT) or MSC depleted of the Snail1 gene (Snail1 KO) as shown by RT-qPCR (left) and western blot (right). (B) A metaphase spread from Snail1 KO MSC showing telomere alterations (SFE, signal-free ends; SA, sister associations). Telomeres were stained with a Cy3-labeled PNA telomere probe (red). (C and D) Frequency of telomere alterations observed in control (CT) and Snail1 KO MSC. N indicates the number of chromosomes analyzed. The mean ± SEM of three independent experiments is shown. *P < 0.05.