Figure 6.

Downregulation of TERRA during EMT correlates with telomere deprotection. (A) NMuMG cells were treated with TGFβ (for 0, 2, 8, or 24 h) to induce EMT. Representative images of NMuMG stained with α-γH2AX (green) and processed for FISH with a Cy3-conjugated (TTAGGG)₃ probe (red) are shown. Co-localizing events (arrowheads) indicate telomere dysfunction-induced foci (TIFs). (B) Quantification of the frequency of cells with > 2 TIFs/nuclei. Between 250 and 300 cells were analyzed in each time point. The value corresponds to the average of three experiments. **P < 0.01; *P < 0.05.