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. 2017 Oct 20;46(1):146–158. doi: 10.1093/nar/gkx958

Figure 7.

Figure 7.

Ectopic TERRA expression alters the EMT gene profile. (A) Western blot of NMuMG cells transduced with Flag-TRF1ΔN alone or fused with the transcriptional activator VP16. (B) Radioactive RNA dot blot for TERRA from NMuMG cells transduced with TRF1ΔN or VP16-TRF1ΔN. 18S served as a loading control. Samples treated with RNase A were used as negative controls. (C) Gene ontology analysis of differentially expressed genes in VP16-TRF1ΔN NMuMG cells as compared to control TRF1ΔN NMuMG cells after TGFβ treatment (24 h). The global transcriptome was analyzed by RNA-seq. (D) Short list of relevant EMT-related genes and their expression in VP16-TRF1ΔN-NMuMG cells as compared to control NMuMG at 24 h after TGFβ treatment. Log2 fold-changes are indicated. A more extended list of transcripts is available in Supplementary Table S2. (E) RT-qPCR was used to determine the expression of the selected EMT-related genes at 24 h after TGFβ treatment (except for Snail1 at 2 h). Data represent the mean ± SEM of three independent experiments. *P < 0.05.