Skip to main content
. 2017 Nov 16;46(1):229–241. doi: 10.1093/nar/gkx1103

Figure 2.

Figure 2.

HPV E1 stimulates pol ϵ. (A) Pol ϵ was added to reactions containing primed M13 template in increasing amounts from 1, 2.5, 5, 7.5 and 10 ng (as indicated, panel B). Pol ϵ was incubated with template alone (lanes 3–8). Pol ϵ was also added in increasing concentrations to reactions containing replication cofactors (RF; RFC (3 ng/μl), PCNA (10 ng/μl), RPA (70 ng/μl; lanes 10–14), or HPV E1 (60 ng/μl; lanes 16–20). RF or E1 alone with no pol ϵ showed no synthesis (lanes 9 and 15, respectively). Lanes 1 and 2 represent a reference panel containing an end-labeled molecular weight marker (M, lane 1) and pol ϵ with RF (lane 2). (B) Synthesis was quantified as in the Methods and graphed, and includes the averages of six experiments. Pol ϵ (10 ng) with RF was set to 100% and all other values were plotted relative to that value. Error bars are defined as s.d. An unpaired student's t-test compared pol ϵ + RF to pol ϵ + E1 at 10 ng of pol ϵ and was not found to be statistically different (lanes 14 and 20; P > 0.05). Compared to pol ϵ alone at 10 ng, results were found to be statistically significant (lanes 8 and 14; P = 0.009).