Figure 5.

HPV E1 confers processivity to pol ϵ. Pol ϵ processivity was examined by performing primed M13 reactions (essentially as in Figure 2) under conditions in which template is in great excess over polymerase (45). Pol ϵ (2.9 fmol) was incubated with E1 (60 ng/μl) increasing concentrations of template (5, 10, 20, and 40 fmoles, lanes 3–7), or with RF with the same increasing concentrations of template (lanes 9–12). A high concentration of pol ϵ (290 fmol) was similarly incubated with the same increasing concentrations of the primed M13 template (lanes 14–17). No template was present in lanes 3, 8 and 13. Lanes 1 and 2 represent a reference marker, as described in Materials and Methods.