Table 2.
MVD assessment.
| Author | MVD assessment method | Antibody | Magnification used | Spots examined | Blinded reading | Observers | Separate count for intra/peritumoral vessels | MVD cut-off |
|---|---|---|---|---|---|---|---|---|
| Döme et al. | Immunohistochemistry, light microscopy, transmission electron microscopy | CD-31, anti-laminin | 20x, 40x | 2 | n/a | n/a | Yes | 30 microvessels/mm2 |
|
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| Hillen et al. | Light microscopy, immunohistochemistry | CD-31, CD-34 | 200x | 4 | Yes | 2 | Yes | n/a |
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| Ilmonen et al. | Light microscopy, immunohistochemistry | CD-31 | n/a | n/a | n/a | n/a | No | 20 microvessels/mm2 |
|
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| Massi et al. | Light microscopy, immunohistochemistry | CD-31 | 10x, 40x | n/a | n/a | n/a | No | Microvessel area: 389.3770/μm2 |
|
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| Pastushenko et al. | Light microscopy, immunohistochemistry, Chalkley score | CD-34 | 100x, 200x, 400x | 3 | n/a | n/a | Yes | 1.67 microvessels/mm2 |
|
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| Ribatti et al. | Light microscopy, immunohistochemistry | VIII factor | 250x | 4–6 | Yes | 2 | No | 10 microvessels/mm2 |
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| Shimizu et al. | Light microscopy, immunohistochemistry | CD-34 | 400x | 4 | Yes | >2 | No | 4 microvessels/mm2 |
|
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| Straume et al. | Light microscopy, immunohistochemistry | VIII factor, CD105 | 25x, 100x, 400x | <10 | n/a | n/a | Yes | 94 microvessels/mm2 |
|
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| Valencak et al. | Light microscopy, immunohistochemistry | CD-31 | 40x, 200x | 3 | Yes | 2 | No | 32.33 microvessels/field |