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. 2018 Jan 4;7:521. doi: 10.3389/fcimb.2017.00521

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Copyright © 2018 Rodrigues, Fernandez, Vercoutere, Chamayou, Andersen, Vigy, Demettre, Seveno, Aprelon, Giraud-Girard, Stachurski, Loire, Vachiéry and Holzmuller.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of A. variegatum saliva on the production of NO and cytokines by LPS-stimulated bovine macrophages. Bovine blood monocyte-derived macrophages were pre-stimulated for 1 h with LPS, and then for an additional 24 h with different concentrations of A. variegatum saliva. Cell culture supernatants were collected and NO production was titrated by the Griess method, IL-10, IL-12, and TNF-α production were titrated by ELISA. Data are means of triplicate experiments, for a pool of saliva batches for the three animals tested. Blue lines and shading show the linear regression fit and the 0.95 confidence interval, respectively. The p-value of the model is indicated on the panel.