Table 1.
Potential immunomodulatory proteins identified in A. variegatum saliva.
| UniProt protein IDs (Leading protein) | Description | Organism | Number of proteins | Peptides | Unique peptides | Sequence coverage [%] | Mol. weight [kDa] |
|---|---|---|---|---|---|---|---|
| IMMUNOGENIC PROTEINS | |||||||
| Q75PV7 | Protein related to Da-p36 | A. variegatum | 1 | 6 | 6 | 29.7 | 27.443 |
| F0J8E1 | Antigen-5 family member (Fragment) | A.variegatum | 1 | 2 | 2 | 32.7 | 12.308 |
| PROTEASE INHIBITORS INCLUDING | |||||||
| Serpins | |||||||
| F0J9G9 | Serpin (Fragment) | A. variegatum | 6 | 11 | 9 | 59.3 | 23.07 |
| F0J8P8 | Serpin (Fragment) | A. variegatum | 1 | 5 | 5 | 57.1 | 17.759 |
| F0J8R0 | Serpin (Fragment) | A. variegatum | 1 | 2 | 1 | 29.1 | 17.208 |
| A0A023GPF9 | Putative tick serpins 14 | A. triste | 6 | 6 | 6 | 14.7 | 44.703 |
| A0A023GN51 | Putative tick serpins 13 | A. triste | 6 | 5 | 1 | 12.1 | 42.841 |
| A0A023GP80 | Putative tick serpins 27 | A. triste | 2 | 3 | 2 | 7.6 | 43.538 |
| A0A023FPT3 | Putative tick serpins 7 (Fragment) | A. cajennense | 1 | 2 | 2 | 7.5 | 35.74 |
| A0A023GGH1 | Putative tick serpins 33 (Fragment) | A. triste | 1 | 2 | 2 | 8.5 | 26.402 |
| A0A023FUL1 | Putative tick serpins 12 (Fragment) | A. cajennense | 3 | 2 | 2 | 7.8 | 46.875 |
| A0A023GPF5 | Putative tick serpins 13 | A. triste | 2 | 2 | 1 | 9.3 | 42.797 |
| G3MLB1 | Putative uncharacterized protein (tick serpins 27) | A. maculatum | 15 | 3 | 1 | 9.6 | 43.103 |
| Other serine protease inhibitors | |||||||
| F0J9Y3 | Kazal-type serine protease inhibitor domain protein | A. variegatum | 4 | 3 | 3 | 20 | 26.71 |
| A0A023GE28 | Putative serine protease inhibitor I. scapularis serine protease inhibitor (Fragment) | A. triste | 2 | 6 | 6 | 18.5 | 46.98 |
| A0A023GE28 | Putative serine protease inhibitor I. scapularis serine protease inhibitor (Fragment) | A. triste | 2 | 6 | 6 | 18.5 | 46.98 |
| A0A023FQ10 | Putative serine protease inhibitor I. scapularis serine protease inhibitor | A. cajennense | 1 | 2 | 2 | 5.1 | 44.715 |
| A0A023GPF6 | Putative serine proteinase inhibitor | A. triste | 2 | 2 | 1 | 8.4 | 43.504 |
| Cysteine protease inhibitors | |||||||
| A0A023FR27 | Putative thyropin | A. cajennense | 6 | 2 | 2 | 12.4 | 22.395 |
| F0JA40 | Putative thyropin | A. variegatum | 7 | 3 | 3 | 12.7 | 22.532 |
| PROTEASES INCLUDING | |||||||
| Serine proteases | |||||||
| F0J9J5 | Trypsin-like serine protease (Fragment) | A. variegatum | 1 | 6 | 6 | 41.1 | 21.867 |
| F0J9E7 | Trypsin-like serine protease (Fragment) | A. variegatum | 3 | 6 | 4 | 48.9 | 19.618 |
| Serine carboxypeptidases | |||||||
| A0A023GNB7 | Putative serine carboxypeptidase lysosomal cathepsin a | A. triste | 1 | 1 | 1 | 5.2 | 53.097 |
| Cathepsin-like cysteine proteases | |||||||
| F0JA27 | Cathepsin L-like cysteine proteinase B | A. variegatum | 8 | 10 | 9 | 36.8 | 37.624 |
| F0J903 | Cysteine proteinase cathepsin L (Fragment) | A. variegatum | 2 | 5 | 5 | 38.6 | 23.017 |
| G3MHB0 | Putative uncharacterized protein (Fragment) (peptidase C1 family; cathepsin l-like cysteine proteinase b) | A. maculatum | 3 | 2 | 1 | 11.5 | 38.867 |
| FERRITIN AND HEMELIPOPROTEINS | |||||||
| G3MPJ3 | Ferritin | A. maculatum | 2 | 3 | 3 | 19.5 | 22.296 |
| F0J8W4 | Heme lipoprotein (Fragment) | A. variegatum | 2 | 33 | 22 | 63.5 | 50.007 |
| F0J9N1 | Heme lipoprotein (Fragment) | A. variegatum | 1 | 20 | 18 | 67.4 | 27.005 |
| F0J9N8 | Hemelipoglycoprotein (Fragment) | A. variegatum | 1 | 14 | 14 | 48.2 | 24.787 |
| F0J9G0 | Heme lipoprotein (Fragment) | A. variegatum | 1 | 11 | 8 | 95.4 | 14.828 |
| A0MVX0 | Heme lipoprotein | A. americanum | 1 | 31 | 5 | 16.3 | 177.04 |
| B7Q406 | Hemelipoglycoprotein. putative | I. scapularis | 2 | 3 | 1 | 1.7 | 177.65 |
| B7QIL0 | Hemelipoglycoprotein. putative (Fragment) | I. scapularis | 1 | 2 | 1 | 1.3 | 172.44 |
| CALCIUM BINDING PROTEINS | |||||||
| F0JA25 | Calreticulin | A. variegatum | 1 | 4 | 1 | 16.3 | 47.318 |
| Q64KA0 | Calreticulin | A. brasiliense | 17 | 4 | 1 | 16.3 | 47.241 |
| G3MK71 | Calponin | A. maculatum | 6 | 3 | 3 | 24.7 | 17.614 |
| FATTY ACID AND HISTAMINE BINDING PROTEIN | |||||||
| A0A023G6C8 | Putative lipocal-1 1 | A. triste | 1 | 1 | 1 | 8.5 | 24.71 |
| A0A023GBM1 | Putative phospholipase a2 I. scapularis phospholipase a2 | A. triste | 2 | 4 | 4 | 11.4 | 44.79 |
| NUCLEIC ACID BINDING PROTEINS | |||||||
| G3MSL8 | Putative uncharacterized protein (Putative gtp-binding protein) | A. maculatum | 10 | 2 | 2 | 12.4 | 24.689 |
| G3MRA1 | Putative uncharacterized protein (gtp-binding protein) | A. maculatum | 4 | 1 | 1 | 6 | 22.968 |
| A0A023FGS1 | Putative zinc-binding protein of the histidine triad hit family | A. cajennense | 3 | 1 | 1 | 11.1 | 13.53 |
| ANTI-OXYDANT ENZYMES | |||||||
| A0A023FIC2 | Putative glutathione s-transferase mu class Rhipicephalus annulatus glutathione s-transferase | A. cajennense | 7 | 6 | 6 | 29.1 | 25.713 |
| A0A023GJM3 | Putative glutathione s-transferase | A. triste | 2 | 2 | 2 | 14.7 | 24.394 |
| A0A023FXR2 | Putative glutathione s-transferase I. scapularis glutathione s-transferase | A. parvum | 5 | 2 | 2 | 10.4 | 26.241 |
| A0A023FFS7 | Putative glutathione s-transferase I. scapularis glutathione s-transferase | A. cajennense | 1 | 1 | 1 | 5.5 | 24.303 |
| A0A023FQ81 | Putative glutathione s-transferase I. scapularis glutathione s-transferase (Fragment) | A. cajennense | 4 | 1 | 1 | 8.9 | 23.244 |
| A0A023G5I8 | Putative glutathione s-transferase I. scapularis glutathione s-transferase (Fragment) | A. triste | 4 | 1 | 1 | 8.6 | 27.412 |
| B7PDV0 | Glutathione S-transferase. putative | I. scapularis | 4 | 1 | 1 | 12.2 | 8.6628 |
| G3MMK0 | Putative uncharacterized protein (glutathione s-transferase I. scapularis glutathione s-transferase) | A. maculatum | 2 | 1 | 1 | 5.9 | 26.159 |
| A0SHR2 | Protein disulfide-isomerase | A. variegatum | 8 | 3 | 3 | 7.2 | 54.923 |
| A0A023FZW2 | Protein disulfide-isomerase | A. parvum | 5 | 4 | 1 | 9.2 | 57.252 |
| A0A023FNM6 | Putative alkyl hydroperoxide reductase thiol specific antioxidant (Fragment) | A. cajennense | 2 | 2 | 2 | 10.7 | 23.542 |
| A0A023FHT1 | Putative thioredoxin peroxidase | A. cajennense | 14 | 4 | 4 | 24 | 20.494 |
| A0A023FDL5 | Putative selenoprotein w 2a | A. cajennense | 1 | 1 | 1 | 22.1 | 8.6959 |
| F0J9H3 | Peroxidasin (Fragment) | A. variegatum | 1 | 1 | 1 | 8.1 | 27.07 |
| A0A023FTX2 | Superoxide dismutase [Cu-Zn] | A. parvum | 1 | 1 | 1 | 9.7 | 15.744 |
| HEAT SHOCK PROTEINS | |||||||
| A0A023GNY6 | Putative heat shock protein (Fragment) | A. triste | 11 | 16 | 13 | 35.6 | 73.124 |
| A0A023GP15 | Putative heat shock protein 90 | A. triste | 9 | 7 | 2 | 13.5 | 84.001 |
| A0A023GLJ3 | Putative heat shock protein 70 | A. triste | 7 | 5 | 2 | 10.5 | 73.014 |
| A0A023FRH0 | Putative heat shock protein | A. cajennense | 1 | 1 | 1 | 5.7 | 21.73 |
| G3MF27 | Putative uncharacterized protein (heat shock 70 kDa protein 5) | A. maculatum | 7 | 4 | 3 | 8 | 69.078 |
| G3MF42 | Putative uncharacterized protein (heat shock protein) | A. maculatum | 2 | 1 | 1 | 7.8 | 21.722 |