Table 2.
Potential ubiquitination complex identified in A. variegatum saliva.
| UniProt protein IDs (Leading protein) | Description | Organism | Number of proteins | Peptides | Unique peptides | Sequence coverage [%] | Mol. weight [kDa] | Propsearch distance (reliability) | Propsearch putative protein family |
|---|---|---|---|---|---|---|---|---|---|
| UBIQUITIN | |||||||||
| F0J9K1 | Ubiquitin (Fragment) | A. variegatum | 12 | 2 | 2 | 29.8 | 9.4959 | – | – |
| E1 UBIQUITIN LIGASE | |||||||||
| A0A023GDS5 | Putative microtubule associated complex (Fragment) | A. triste | 2 | 12 | 9 | 14.1 | 117.06 | 5.38 (99.6%) | Ubiquitin-activating enzyme E1 (A1S9 protein). |
| A0A023G500 | Putative nidogen (Fragment) | A. triste | 1 | 1 | 1 | 1.2 | 124.2 | 6.21 (99.6%) | Ubiquitin-activating enzyme E1 homolog (D8) |
| B7QMC8 | Alpha-macroglobulin. putative (Fragment) | I. scapularis | 1 | 8 | 1 | 7 | 121.21 | 4.83–5.37 (99.6%) | Ubiquitin-activating enzyme E1 |
| B7PN44 | Netrin-4. putative | I. scapularis | 1 | 2 | 2 | 5.1 | 41.593 | 7.21 (99.6%) | Ubiquitin-activating enzyme E1 X (Fragment) |
| B7PQH0 | Hemolectin. putative (Fragment) | I. scapularis | 1 | 2 | 2 | 2.4 | 115.88 | 5.66–5.67 (99.6%) | Ubiquitin-activating enzyme E1 |
| A0A023GPI0 | Uncharacterized protein (Fragment) | A. triste | 3 | 28 | 1 | 32.3 | 131.52 | 4.90–5.42 (99.6%) | Ubiquitin-activating enzyme E1 |
| E2 UBIQUITIN LIGASE | |||||||||
| B5M727 | Alpha-2-macroglobulin (Fragment) | A. americanum | 2 | 5 | 2 | 42.6 | 19.026 | 8.69–10.05 (94% > x > 80%) | Ubiquitin-conjugating enzyme E2-24 kDa (EC 6.3.2.19) |
| A0A023G356 | Putative secreted protein (Fragment) | A. triste | 1 | 3 | 3 | 12.2 | 21.619 | 11.17 (80%) | Ubiquitin-conjugating enzyme E2-24 kDa (EC 6.3.2.19) |
| A0A023FDX3 | Putative I. scapularis glutathione peroxidase (Fragment) | A. cajennense | 1 | 1 | 1 | 12.8 | 10.918 | 11.13–12.43 (80% > x > 68%) | Ubiquitin-conjugating enzyme variant MMS2 homolog (UEV MMS2) |
| G3MNL6 | Putative uncharacterized protein (alpha crystallins?) | A. maculatum | 2 | 1 | 1 | 13.1 | 19.912 | 9.69 (87%) | Ubiquitin-conjugating enzyme E2-24 kDa (EC 6.3.2.19) |
| E2/E3 UBIQUITIN LIGASE | |||||||||
| B7P1U8 | Spectrin alpha chain. putative | I. scapularis | 4 | 4 | 4 | 2 | 272.3 | – | – |
| DE-UBIQUITINATING ENZYME | |||||||||
| A0A023GME3 | Putative vitellogenin-1 (Fragment) | A. triste | 1 | 38 | 10 | 28.4 | 153.63 | 6.12–7.44 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 15 (EC 3.1.2.15) |
| A0A023GMC7 | Putative vitellogenin-1 (Fragment) | A. triste | 2 | 34 | 6 | 30.6 | 123.4 | 6.42–6.80 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 15 (EC 3.1.2.15) |
| A0A023GP74 | Putative titin (Fragment) | A. triste | 2 | 21 | 15 | 10.4 | 389.34 | 7.13 (99.6%) | Probable ubiquitin carboxyl-terminal hydrolase FAF (EC 3.1.2.15) |
| A0A023G0F9 | Putative peroxinectin I. scapularis peroxinectin (Fragment) | A. parvum | 1 | 16 | 3 | 32.1 | 87.425 | 5.42 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 20 (EC 3.1.2.15) |
| A0A023GCH5 | Putative neurexin iii-alpha (Fragment) | A. triste | 1 | 9 | 9 | 8.4 | 158.55 | 5.72 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 19 (EC 3.1.2.15) |
| A0A023GLZ9 | Putative extracellular matrix glycoprotein laminin subunit beta (Fragment) | A. triste | 1 | 9 | 4 | 15.7 | 92.371 | 5.25 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 20 (EC 3.1.2.15) |
| B7P403 | Type II transmembrane protein. putative | I. scapularis | 1 | 6 | 6 | 2.6 | 319.6 | 5.79 (99.6%) | Probable ubiquitin carboxyl-terminal hydrolase FAF (EC 3.1.2.15 |
| B7PUM7 | Peroxinectin. putative | I. scapularis | 1 | 4 | 1 | 6.7 | 90.84 | 5.18 (99.6%) | Ubiquitin carboxyl-terminal hydrolase 20 (EC 3.1.2.15) |