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. Author manuscript; available in PMC: 2018 Jan 9.
Published in final edited form as: Toxicol Appl Pharmacol. 2016 Sep 20;311:106–112. doi: 10.1016/j.taap.2016.09.016

Fig. 4.

Fig. 4

Apigenin suppresses lung metastasis through downregulation of NEDD9. Athymic nude mice were injected with DLD1 cells with or without stable knockdown of NEDD9 via tail vein. The mice were i.p. treated with apigenin (APG) (20 mg/kg) once per week. The lungs were isolated at week 14. The total number of metastatic lesions was counted (n = 7–8 mice per group). (A) The number of metastatic foci in the lungs of each mouse. * p < 0.05 compared to scramble control cells without APG treatment. (B) The proportion of mice with metastasis in each group. (C) Representative H&E stained lung section. Arrows indicate metastatic foci in the lungs. (D) Representative immunohistological staining for expressions of NEDD9 and CK20 in the metastatic foci. Arrows indicate metastatic DLD1 cells in the lung section.