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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Pediatr Blood Cancer. 2017 Sep 5;65(1):10.1002/pbc.26721. doi: 10.1002/pbc.26721

Figure 4. Twist1 is required for Ewing Sarcoma metastasis.

Figure 4

IHC of Twist1 from primary tumors show persistent Twist1 knockdown in the xenograft model in both A673 (A) and TC32 Ewing sarcoma xenografts (C). Fluorescent images of lung metastases from A673 and TC32 xenograft tumors. Arrows point to GFP positive Ewing sarcoma metastases (A and C). B Primary tumor weight of A673 xenograft tumors expressing control or Twist1 shRNAs (p = ns, no significance) Quantification of lung metastases from A673 xenograft tumors (*p< 0.0016, **p<0.0026, T-test, n=10 mice in each group). D. Primary tumor weight of TC32 xenograft tumors expressing control or Twist1 shRNAs (p =ns). Quantification of lung metastases from TC32 xenograft tumors (*p< 0.09, **p<0.027, T-test, n=5 mice in each group).