Figure 6. Twist1 overexpression promotes migration and invasion of RDES cells.
A. Left, lysates from RDES-parental, RDES-GFP and RDES-Twist1 cells were analyzed by SDS-PAGE and probe for Twist1 and GAPDH. Right, bright-field images of RDES cells expressing GFP or Twist1. Migration (B) and invasion (C) assay of RDES-parental, RDES-GFP and RDES-Twist1 cells. Representative images of migrated and invasive cells stained with crystal violet and quantification of relative migration and invasion for one representative experiment. Data were presented as meanĀ±SEM. **P<0.01 by ANOVA followed by Bonferroni test.