Skip to main content
. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Glia. 2017 Sep 14;66(1):108–125. doi: 10.1002/glia.23231

Figure 2. AQP4 co-associates more with TRPM4 than with TRPV4.

Figure 2

(A) Anti-myc immunoblots of direct lysate input and AQP4 immunoprecipitation (IP) in COS-7 cells expressing AQP4, myc-TRPM4, and myc-TRPV4, showing equivalent abundance of myc-TRPM4 and myc-TRPV4 in direct lysate, but markedly greater abundance of myc-TRPM4 versus myc-TRPV4 upon IP of AQP4 M1 or M23 isoforms; displayed immunoblot includes all (n=3) independent experimental replicates. (B) Densitometric quantification of the data in panel (A), showing that the AQP4/myc-TRPM4 complex is 5–14 times more abundant than the AQP4/myc-TRPV4 complex; *p<0.05 in t-test between groups; n=3 independent experiments.