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. 2018 Jan 9;51:3. doi: 10.1186/s40659-017-0151-6

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Morphology and cellular composition of duodenal tissue. (a–c) Haematoxylin and eosin (H&E)-stained sections from control (a), short-term gavage, 50 mg G007-LK/kg body mass (b), and long-term enriched chow, 100 mg G007-LK/kg chow (c) treated mice. d, e Periodic acid-Schiff (PAS)-stained cells (indicated with black arrows) were identified in duodenal tissue samples from control (d) and G007-LK (e) treated (gavage 50 mg G007-LK/kg body mass) mice (inset shows enlargements of boxed areas in main panel). f Quantification of the average number of PAS-positive cells per villus in duodenal tissue sections from control and G007-LK-treated mice (mean ± SEM, n = 3). g, h IHC of duodenal tissue sections, stained with an antibody against Lysozyme, from control (g) and G007-LK treated, gavage 10 mg G007-LK/kg body mass (h) mice (inset shows enlargements of boxed areas in main panel). (i, j) IHC of duodenal tissue sections, stained with an antibody against Chromogranin A (positive cells indicated with black arrows), from control (i) and G007-LK-treated, long term enriched chow, 100 mg G007-LK/kg chow (j) mice (inset shows enlargements of boxed areas in main panel)