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. 2018 Jan 9;49:3. doi: 10.1186/s13567-017-0501-y

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Wild-type and capsule or MeOPN deficient strains C. jejuni induce similar cytokine responses in huMDMs. Live or fixed C. jejuni 11168H (WT), Δcj1417 (MeOPN) and Δcj1439 (capsule) mutants were incubated with huMDMs at MOI 50 for 3 h. Salmonella LPS was used as a positive control, and mRNA levels of target gene was presented as folder changes compared to PBS in the medium (a negative control). Graphs show representative results of three individual donors (two individuals for fixed C. jejuni). Error bars are standard deviation of three (two) independent experiments. Asterisks indicate a statistically significant difference of mRNA levels for the indicated inflammation-related cytokines compared to PBS in the medium (*P < 0.01).