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. 2018 Jan 9;17:3. doi: 10.1186/s12934-017-0853-z

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Overlay of trastuzumab displaying yeast cells either stained with detection antibodies only (blue) or with detection antibodies and HER2 as monitored by flow cytometry. Yeast cells were consecutively incubated with 1 µM of His tagged HER2 followed by secondary labeling with Alexa Fluor 647 conjugated anti-Penta-His antibody (target binding) and PE conjugated anti-kappa-antibody (display)