Table 3.
Haematological and biochemical findings for 10 heifers after first real-time PCR positive result for A. phagocytophilum
| Parameter | Reference values | Days after first positive real-time PCR result | |||||
|---|---|---|---|---|---|---|---|
| 0 | 7 | 14 | 21 | 28 | 200 | ||
| Leukocytes | 4–10 G/l | 3.55 (1.9–6.2)* | 5.8 (2.9–9.8) | 6.95 (3.3–10.6) | 6.85 (2.9–19.5) | 6.95 (4.0–11.3) | 8.4 (6.5–14.4) |
| Erythrocytes | 5–8 T/l | 6.16 (4.8–9.1)* | 6.12 (4.9–8.3) | 6.495 (5.3–9.3)* | 6.135 (4.9–8.6)* | 6.42 (5.0–9.2) | 9.51 (6.3–11.6) |
| Haemoglobin | 10–13 g/dl | 9.75 (7–15.1) | 9.3 (7.4–14.8) | 10.25 (8.2–14.5)* | 9.35 (8.3–14.3)* | 9.75 (7.8–15.1) | 14.4 (9.4–17.6) |
| Haematocrit | 30–36% | 29.8 (22.3–49.3) | 29.7 (24.9–45.9) | 33.05 (26.5–47.5) | 30.2 (26.5–46.6) | 31.4 (25.4–48.4) | 40.6 (29.9–49.7) |
| Thrombocytes | 200–800 G/L | 87 (22–333) | 192 (100–436) | 276.5 (61–584) | 312 (96–513)* | 338.5 (28–484) | 175 (94–421) |
| Banded neutrophils | 0–0.1 G/l | 0.24 (0–0.6) | 0.23 (0.1–1.8) | 0.405 (0.07–1.04) | 0.165 (0.04–5.66) | 0.445 (0.05–1) | 0.245 (0–1.68) |
| Seg. neutrophils | 0.85–1.53 G/l | 1.52 (0.9–3.8) | 1.98 (0.7–3.5) | 2.555 (0.8–5.15) | 2.435 (1.3–9.6) | 3.615 (1.1–5.5) | 4.18 (3.1–8.4) |
| Lymphocytes | 2.5–5.5 G/l | 1.09 (0.6–2.4)* | 3.2 (0.9–5.2) | 3.62 (0.91–5.1) | 3.025 (1.16–6.79) | 3.705 (1.08–6.14) | 2.46 (1.56–4.3) |
| Monocytes | 0–0.2 G/l | 0.02 (0–0.32)* | 0.17 (0–0.69) | 0.225 (0.03–0.9) | 0.205 (0–0.47) | 0.24 (0.04–0.77) | 0.2 (0.07–0.65) |
| Eosinophils | 0–0.9 G/l | 0.05 (0–0.68) | 0.06 (0–2.8) | 0.11 (0–0.42)* | 0.17 (0–1.19) | 0.23 (0–0.98) | 0.55 (0–2.3) |
| Basophiles | 0–0.1 G/l | 0 (0–0) | 0 (0–0) | 0 (0–0) | 0 (0–0.11) | 0 (0–0.11) | 0 (0–0.12) |
| Urea nitrogen | ≤5.,5 mmol/l | 6.25 (2.6–9.8) | 4.75 (2.7–6) | 4.4 (2.8–7) | 4.65 (1.9–6.5) | 4.5 (1.4–7.7) | 4.65 (3.8–8.2) |
| Creatinine | ≤110 μmol/l | 80.83 (59.0–133.8)* | 68.645 (58.1–119.9) | 79.54 (61.6–115.2)* | 63.605 (50.9–110.6) | 71.265 (54.5–97.6) | 57.105 (10–96.9) |
| Total protein | 40–80 g/l | 75.65 (64.9–91.4) | 75.7 (66.9–90.8) | 75.95 (67.5–88.1) | 74.1 (66–88.5) | 74.3 (62.2–89.4) | 75.25 (64.5–81.9) |
| Albumin | ≤40 g/l | 33.65 (28.9–38.7)* | 34.15 (21.9–41)* | 33.2 (28.6–45.1)* | 32.85 (23.4–40.9)* | 34.6 (28.2–39.6)* | 37.3 (29.9–40.4) |
| Bilirubin | ≤8.5 μmol/l | 3.885 (1.7–14.3)* | 2.27 (1.5–5.5)* | 1.7 (0.7–2.52)* | 2.04 (0.46–14.6)* | 1.74 (0.91–5.12)* | 0.815 (0.11–1.43) |
| AST | ≤80 U/l | 74.45 (48.6–121.8) | 85.95 (56.5–130.7) | 74.85 (62.9–95.2) | 76.6 (58–110.7) | 76.05 (64.7–108.3) | 89.15 (59.2–146.9) |
| γ-GT | ≤36 U/l | 16.35 (8.4–23.6)* | 21 (9.8–32.1)* | 19.4 (12.8–63.3)* | 21 (13.1–64.4) | 17.65 (10.3–75.2)* | 38 (29.4–71) |
| GLDH | ≤16 U/l | 5.345 (3.3–26.5)* | 5.6 (2.6–26.3) | 5.915 (4.1–13.7)* | 5.77 (3.5–20.6)* | 7.43 (1.3–26.9)* | 15.13 (10.09–47.52) |
| CK | ≤245 U/l | 244 (95–824) | 142 (70–710) | 150.5 (78–387) | 159.5 (79–1538) | 193 (97–480) | 154 (105–795) |
| P | 1.5–3 mmol/l | 1.5 (1.1–3.2) | 2.1 (1.3–2.7) | 2.35 (1.7–2.7) | 2.25 (1.6–2.9) | 2.2 (1.7–2.8) | 2.2 (1.9–2.4) |
| Mg | 0.74–1.44 mmol/l | 0.78 (0.73–1.08)* | 0.9 (0.73–1.01)* | 0.93 (0.75–1.07)* | 0.94 (0.72–1.01) | 0.98 (0.82–1.06) | 1.005 (0.9–1.12) |
| Ca | 2–3 mmol/l | 2.045 (0.97–2.34)* | 2.17 (1.94–2.4)* | 2.23 (1.92–2.38)* | 2.245 (2.09–2.4)* | 2.285 (1.99–2.43)* | 2.455 (2.1–2.71) |
| Sodium | 130–150 mmol/l | 135 (131–139) | 137.5 (135–140) | 138 (133–143) | 138 (134–140) | 137.5 (135–140) | 140 (131–144) |
| Potassium | 3.5–5.3 mmol/l | 4.58 (3.8–5.9) | 4.605 (4.0–5.5)* | 4.845 (4.4–6.1) | 5.19 (4.4–5.7) | 4.985 (4.4–6.6) | 5.27 (4.78–6.01) |
| Chloride | 98–106 mmol/l | 98 (90–102) | 97 (91–100) | 95 (93–99) | 96 (92–102) | 96 (92–100) | 97 (93–104) |
Note: Values are medians (minimum–maximum)
*P < 0.01 (Wilcoxon signed-rank test for paired samples)