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RR-RR GP64 mutant enhances entry into HAE. wtGP64-FIV-gaussia luciferase (gLuc) and RR-RR GP64-FIV-gLuc were applied to the apical surface of polarized HAE at multiplicity of infection (MOI) of 0.5, 5 and 50 (n = 9) (a) and pig airway epithelia at an MOI of 50 (n =9) (b) overnight. Apical washes were collected 4 days post transduction, and relative light unit (RLU) intensity of Gaussia luciferase was measured and normalized to wtGP64.
