TABLE 1.

Primary Antibodies Used

Antigen	Immunogen	References	Source, catalog No., lot No.	Host species	RRID	Dilution
Synaptotagmin-2	1–5 day zebrafish embryo	Fox and Sanes, 2007; Cooper and Gillespie, 2011	Zebrafish International Resource Center, catalog No. ZDB-ATB-081002-25	Mouse monoclonal, IgG2a	AB_10013783	1:250
Synaptophysin clone Z66	C-terminus of human synaptophysin	Lai et al., 2008; Harrington et al., 2012	Molecular Probes, catalog No. 80130	Rabbit polyclonal	AB_2315414	1:100
Pan-axonal neurofilament	Nonphosphorylated neurofilament H in homogenized rat brain	Sternberger et al., 1982; Petralia et al., 2011	Covance Research Products, catalog No. SMI-312	Mouse monoclonal, IgG1	AB_2314906	1:1,000
Vesicular acetylcholine transporter (VAChT)	Linear peptide; (CEDDYNYYSRS) corresponding to the C-terminus of rat VAChT	Duplan et al., 2010; Enjin et al., 2010; Wootz et al., 2013	Millipore, catalog No. AB1588, lot No. 2167122	Guniea pig polyclonal	AB_11214110	1:250
Vesicular glutamate transporter (VGluT1)	Strep-tag fusion containing amino acids 456–560 of rat VGluT1	Garbelli et al., 2008; Balaram et al., 2015	Synaptic Systems, catalog No. 135303	Rabbit polyclonal	AB_887876	1:250
NeuN	N-terminus of GST-tagged recombinant protein of cell nuclei purified from mouse brain	Mullen et al., 1992; Scott and Lois, 2007	Millipore, catalog No. MAB377, lot No. 2159655	Mouse monoclonal, IgG1	AB_2298772	1:250