Fig. 1. Protein Changes in DIV10 Cortical Neurons Induced by Maintaining Cultures in Different Medium & Plating Conditions.

(a) Representative total membrane stain showing equal protein loading and membrane transfer across groups. (b) Diagram illustrating time course of culture maintainance in different media treatment groups. Western blots show differences in levels of (c) cell-type specific markers NeuN and GFAP, (d) proteins invovled in neurotransmission including GluN2B, GluR1, and CREB, (e) proteins involved in axonal development including NF-H, NF-L, Tau, and PP2A. (f) Examples in the literature of each target and their importance in the setting of TBI research. (g) Western blot shows stimulation of synaptic activity-induced ERK activiation with 4AP/Bicuculline in DIV9 neurons maintained in Neurobasal®/B27 or BrainPhys®/SM1.