Fig. 2. The Effect of Medium & Plating Conditions on DIV6 Cortical Neuron Adherance and Morphology on Deformable Silastic Membranes.

DIV6 neurons were grown on Silastic membranes and analyzed by immunofluroresence to detect NeuN (Red) and Tau (Green). (a–b) Secondary only controls show absence of Red/Green signal at equivalent exposures used in subsequent panels. (c) DAPI+ staining (Blue) confirm presence of cells in secondary only controls. (d–f) Detection of NeuN (Red) and Tau (Green) in Neurobasal®/B27 maintained neurons grown on PDL coated Silastic membranes. Cells easily detached under these condtions. (g–i) Detection of NeuN (Red) and Tau (Green) in Neurobasal®/B27 maintained neurons grown on Matrigel coated Silastic membranes. Cells more readily attach under these conditions, and tend to show a lattice-like pattern. (j–l) Detection of NeuN (Red) and Tau (Green) in Neurocult®/BrainPhys®/SM1 maintained neurons grown on Matrigel coated Silastic membranes. Cells strongly attach under these conditions and axons (large-caliber and small) are widespread. Images show 10X magnification.