Fig. 4. NF-L Staining After a Single or Multiple Stretch-Injuries.
DIV9 cortical neurons were given a single or multiple 54% biaxial stretch-injuries. Immunofluorescence was done to detect the neuronal marker NeuN (Red) and axonal marker NF-L (Green) 24h later. (a, c, and e) Overlay of nuclear staining NeuN+ neuronal clusters with nuclear DAPI (Blue) in uninjured controls, 1X stretch-injured neurons, and 2X stretch-injured neurons. (b, d, and f) Co-localization of NeuN+ neuronal clusters with NFL+ somata/axons in uninjured controls, 1X stretch-injured neurons, and 2X stretch-injured neurons. Images show 10X magnification.