Fig. 1.

Epoxyazadiradione a neem limonoid exhibits potent cytotoxic effect in breast cancer cells: a Structure of ten major limonoids isolated from neem. b MDA-MB-231 cells were seeded into 96-well plate at density of 1 × 10⁴ cells and treated with ten major neem limonoids (1: Epoxyazadiradione; 2: Azadiradione; 3: 17β-hydroxyazadiradione; 4: Gedunin; 5: Nimbin; 6: 6-Deacetylnimbin; 7: Salannin; 8: 3-Deacetylsalannin; 9: Azadirachtin A; 10: Azadirachtin B) for 24 h at 100 and 200 μM and cell death was analyzed using MTT assay. The inhibition of the percentage of cell viability is represented into bar graph. Values are represented in mean ± SEM of three independent experiments