Fig. 5.

WWL70 treatment attenuated both mRNA and protein level of pro-inflammatory cytokines and chemokines in the ipsilateral sciatic nerve of mice after CCI. RNA isolated from sciatic nerves removed from mice 7 days post-CCI surgery were subjected to qRT-PCR analysis. CCL2 (a), IL-1β (b), and IL-6 (c) expressions were significantly elevated after CCI and reduced by WWL70 at 10 mg/kg, but not at 5 mg/kg treatment. *p < 0.05 and **p < 0.01 CCI/WWL70 (10 mg/kg) compared to CCI/vehicle group (mean ± S.E.M., n = 10/group). The production of CCL2 (d) and IL-1β (e) in sciatic nerve was measured by ELISA and shown to be significantly increased in the vehicle group compared to the control group (*p < 0.05 and **p < 0.01) and dramatically reduced by WWL70 (10 mg/kg). (*p < 0.05, mean ± S.E.M., n = 5/group)