Table 1.
Sequence and melting temperature of ODNs
| X | Y | Tm (°C)a,b,c (ΔTm based on T control) |
|---|---|---|
| ODN1: 5′-ATCGCAXGTA-3′ | ||
| ODN4: 3′-TAGCGYACAT-5′ | ||
| T | T | 43.5 ± 1.1 |
| 5fU | 5fU | 45.2 ± 0.1 (1.7) |
| ODN2: 5′-CGXACXAGXACG-3′ (self-complementary) | ||
| T | — | 51.6 ± 0.4 |
| 5fU | — | 52.5 ± 0.4 (0.9) |
| U | — | 50.1 ± 0.4 (−1.5) |
| 5hmU | — | 47.8 ± 0.1 (−3.8) |
| ODN3: 5′-GAACXGXCXGAG-3′ | ||
| ODN5: 3′-CTTGACAGACTC-5′ | ||
| T | — | 49.8 ± 0.3 |
| 5fU | — | 49.4 ± 0.1 (−0.4) |
| U | — | 51.1 ± 0.2 (1.2) |
| 5hmU | — | 46.7 ± 0.1 (−3.1) |
| base J | — | 47.9 ± 0.2 (−1.9) |
a
Mean ± sd values of three measurements are shown.
b
Tm was measured at 5 μM of each ODN in PBS.
c
See the ESI for melting curves.