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The effect of change in temperature from (a) 25 °C to 37 °C, and from (b) 37 °C to 25 °C on the attenuation spectrum of Definity® measured over 60 minutes, (c) The mean attenuation coefficient of Definity® warmed from 25 °C to 37 °C, and cooled from 37 °C to 25°C versus time (n = 3).