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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Exp Neurol. 2017 Nov 16;300:179–187. doi: 10.1016/j.expneurol.2017.11.010

Figure 2. Flow cytometry gating strategy to differentiate between T cells, resident microglia, and infiltrating monocytes.

Figure 2

Flow cytometric analysis of ventral midbrain isolates and gating scheme for isolating mononuclear cells from Naïve, AAV2-GFP, and AAV2-SYN transduced red/green mice. Mononuclear cells were gated on forward (FSC) and side scatter (SSC), live cells (Live/Dead), and CD45+ expression. Gating was designed to distinguish two distinct populations of cells: (Myeloid) a CD45+ CD11b expressing population which are myeloid, and (D) a CD45+, CD4 expressing population which are T cells. (A) Isolation of the CD45+, CD11b+ myeloid population reveals (B) two distinct gates of resident microglia which are CX3CR1-GFPhi and infiltrating monocytes which are CCR2-RFP+. (C) Further gating of CCR2-RFP+ monocytes indicate that the monocytes are Ly6C+. Cells that were Ly6G+ were excluded from analysis (not shown).