Table 6. Hub genes identified in the cluster-based networks and their associated microRNAs.
| microRNA | mRNA | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cluster | MicroRNA connected to hub gene | Change | Expression p-value | Time DE | MicroRNAs dysregulated in PFC of human alcoholics and/or alcohol-dependent rats |
Hub gene | Change | Expression p-value | Time DE | Species and mRNA reference link |
| AMY Cluster1 | miR-181a-5p | ⬇ | 2.71E-02 | 0h | - | Bcl2 | ⬆ | 9.16E-03 | 120h | Rat (Ref) |
| miR-34a-5p | ⬇ | 1.93E-02 | 0h | - | ||||||
| AMY Cluster2 | miR-125b-5p | ⬆ | 4.44E-02 | 0h | rno-miR-351_st | Bcl2 | Same as above | |||
| AMY Cluster3 | miR-2861 | ⬆ | 2.73E-02 | 0h | - | Map2k7 | ⬇ | 7.64E-03 | 120h | Human (Ref) |
| miR-3960 | ⬆ | 4.91E-02 | 0h | - | ||||||
| miR-486-3p | ⬆ | 4.15E-02 | 0h | - | ||||||
| AMY Cluster4 | miR-16-5p | ⬇ | 1.58E-02 | 0h | rno-miR-195_st/hsa-miR-15b-5p | Bcl2 | Same as above | |||
| miR-17-5p | ⬇ | 3.25E-02 | 0h | rno-miR-93_st | ||||||
| +miR-503-5p | - | - | - | - | ||||||
| AMY Cluster5 | +mir-322 | - | - | - | - | Bcl2 | Same as above | |||
| miR-16-5p | ⬇ | 1.98E-02 | 8h | rno-miR-195_st/hsa-miR-15b-5p | ||||||
| NAC Cluster1 | miR-146a-5p | ⬇ | 4.05E-02 | 0h | hsa-miR-146a-5p | Mapt | ⬇ | 4.05E-02 | 120h | Rat (Ref) |
| NAC Cluster2 | miR-34a-5p | ⬆ | 4.22E-02 | 0h | hsa-miR-34c-5p | Hnf4a | ⬇ | 4.58E-02 | 120h | Rat (Ref) |
| NAC Cluster3 | miR-3118 | ⬆ | 4.44E-02 | 0h | - | Egfr | ⬆ | 2.91E-03 | 8h | Drosophila (Ref) |
| NAC Cluster4 | miR-7a-5p | ⬆ | 1.63E-02 | 120h | rno-miR-7a_st/hsa-miR-7-5p | Egfr | Same as above | |||
| NAC Cluster5 | miR-129-1-3p | ⬆ | 2.64E-02 | 8h | rno-miR-129-star_st | Bag3 | ⬆ | 2.76E-02 | 120h | Human (Ref) |
| PFC Cluster1 | miR-141-3p | ⬆ | 2.79E-02 | 0h | - | Ctnnb1 | ⬇ | 4.56E-02 | 120h | Avian (Ref) |
| PFC Cluster2 | miR-141-3p | ⬆ | 4.19E-02 | 0h | - | Ctnnb1 | Same as above | |||
| miR-155-5p | ⬆ | 2.22E-03 | 0h | - | ||||||
| PFC Cluster3 | miR-101-3p | ⬇ | 1.81E-02 | 0h | rno-miR-101b_st/hsa-miR-101-3p | Srf | ⬆ | 9.14E-04 | 120h | Ferrets (Ref) |
| miR-329-3p | ⬇ | 4.00E-02 | 0h | - | ||||||
| miR-335-5p | ⬆ | 3.98E-02 | 120h | rno-miR-335_st | ||||||
| PFC Cluster4 | miR-129-5p | ⬆ | 4.38E-02 | 8h | - | Fndc3b | ⬆ | 4.77E-02 | 120h | Human (Ref) |
| miR-188-5p | ⬇ | 1.80E-02 | 120h | - | Rbfox2 | ⬆ | 2.54E-03 | 8h | Human (Ref) | |
| miR-34a-5p | ⬇ | 3.06E-02 | 0h | hsa-miR-34c-5p | Sh3kbp1 | ⬆ | 3.08E-02 | 120h | Mouse (Ref) | |
| PFC Cluster5 | miR-92a-3p | ⬆ | 3.12E-02 | 8h | rno-miR-92b_st/hsa-miR-92a-3p | Sdhd | ⬇ | 1.91E-02 | 120h | Human (Ref) |
| PFC Cluster6 | miR-3093-3p | ⬆ | 4.58E-02 | 8h | - | Srf | Same as above | |||
| miR-486-5p | ⬆ | 9.37E-03 | 120h | - | ||||||