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. 2018 Jan 9;13(1):e0190655. doi: 10.1371/journal.pone.0190655

Fig 1. Endogenous C3a does not promote anti-MPO glomerulonephritis but supports macrophage recruitment to the inflamed glomerulus.

Fig 1

Anti-MPO glomerulonephritis was induced in WT and C3ar-/- mice (n = 14/group, data from two independent experiments) by injection of 100μg/g anti-MPO IgG and 0.5μg/g LPS. On day 7 histological glomerular injury was assessed. Both groups of mice developed glomerulonephritis including segmental necrosis. There was no difference in degree of injury (A) between WT (B) and C3ar-/- (C) mice. Glomerular leucocyte influx was assessed by immunohistochemistry (n = 7/group). Compared to WT mice (D-E), the number of glomerular macrophages was reduced in C3ar-/- mice. (F) There was no difference in glomerular neutrophils (G), Albuminuria, measured by urinary albumin: creatinine ratio was not different between groups (H). ** p<0.01 by Mann-Whitney U Test.