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. 2018 Jan 9;13(1):e0190655. doi: 10.1371/journal.pone.0190655

Fig 2. Endogenous C3a does not promote autoimmune anti-MPO glomerulonephritis.

Fig 2

Autoimmunity to MPO was induced in WT and C3ar-/- mice (n = 13-15/group, data from two independent experiments) by immunisation with 20μg MPO in FCA followed 7 days later by 10μg MPO in FIA. Disease was triggered on day 16 by i.v. injection of 0.12mg/g sheep anti-mouse GBM globulin in two divided doses and glomerular injury was assessed on day 21. Mice developed glomerulonephritis with focal areas of segmental necrosis, but there was no difference in the degree of injury (A) between WT (B) and C3ar-/- (C) mice. Glomerular leucocytes were assessed by immunohistochemistry. The number of glomerular CD4+ cells (D), macrophages (E) and neutrophils (F) were similar between groups. Functional renal injury measured by urinary albumin: creatinine ratio was similar between groups (G).