Fig 4. C3ar^-/- mice have normal B cell development in the spleen and bone marrow.

Bone marrow cells were extracted from tibiae of naïve WT and C3ar^-/- mice (n = 4/group) and surface markers analysed by flow cytometry. (A-C) The proportion of B220⁺ cells that were preB or proB (IgM^lowIgD^low), transitional (IgM⁺IGD^low) or mature (IgM⁺IgD⁺) did not differ between groups. (D) Representative flow cytometry plot gated on B220⁺ cells in the bone marrow showing gating strategy. The splenic B cell compartment was analysed in WT and C3ar^-/- mice (n = 8/group) at the end of the autoimmune anti-MPO glomerulonephritis model. There was no difference in (E) the proportion of splenocytes that were B220⁺ B cells, (F) the proportion of B220⁺ cells that were CD21⁺CD23⁺ follicular B cells, or (G) CD21⁺CD23^low marginal zone B cells was similar between groups. (H) The proportion of splenocytes that were B220^low/intCD138⁺ plasma cells also did not differ between groups. (I) Representative flow cytometry plot of splenocytes showing gating of plasma cells. (J) Serum B cell activating factor (BAFF) was similar between groups.