Fig 5. Endogenous C3a does not promote early cellular immunity to myeloperoxidase.

To assess the early cellular immune response lymphocytes from the draining lymph nodes of WT and C3ar^-/- mice (n = 8/group) were studied 10 days after immunisation with 20 μg MPO in FCA. There was no difference between groups in Th1 and Th17 response measured by (A) IFN-γ and (B) IL-17A ELISPOT. (C) The proportion of activated CD44⁺CD4⁺ T cells was similar between groups. (D) Representative flow cytometry plots gated on CD4⁺ cells showing CD25⁺Foxp3⁺ Tregs. (E) The proportion of CD4 cells that were Tregs did not differ between groups. (F) Representative flow cytometry plots gated on CD4⁺ cells showing PD-1^hi CXCR5^hi T follicular helper cells. (G) There was no difference between groups in the proportion of CD4 T cells that had a TFH phenotype.