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. 2017 Dec 27;13(12):e1007131. doi: 10.1371/journal.pgen.1007131

Fig 10. Immunoblot analysis of IniRMtb with α-Strep antibody.

Fig 10

M. smegmatis containing the construct pEXCF-iniRMtb-Strep vector was cultured and cultures were exposed for 18 hours to 10 ng/ml ATc/ Strep-tagged IniRMtb was isolated with StrepTactin beads. Purified protein from the elution fractions (elution 1–5, see S6 Fig) was exposed to either trehalose, ATP, both or neither. Presence is indicated with (+), absence is indicated with (-). Indicated on the image of the Western Blot are monomeric IniR (~90kDa) and possible dimers and tetramers.