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. 2017 Dec 27;13(12):e1007148. doi: 10.1371/journal.pgen.1007148

Fig 5. σ32 inhibits growth in a dose-dependent manner.

Fig 5

(A) Growth curves of the wild type and PdnaK::Tn strain harboring either the empty vector pJS14 or a plasmid for the vanillate-inducible overexpression of rpoH, rpoH(V56A), rpoH(V56A; Q202R) or rpoH(V56A; YWW::AAA). Growth was monitored in the absence or presence of vanillate. (B) LacZ assays monitoring σ32-dependent expression from the dnaKJ promoter region on the reporter plasmid pDel1 in the otherwise same strain backgrounds and under the same conditions as shown in (A). (C) Growth curves of the PdnaK::Tn strain harboring a plasmid allowing for combined overexpression of rpoD and rpoH. Vanillate induces the expression of rpoH, xylose induces the expression of rpoD. All experiments were performed as independent duplicates. Error bars represent standard deviations.