Fig 7. The disassociation of LSD1 from IFITM3 by IAV and VSV infections can counteract IFITM3.

(A)Virus infection reduces the interaction between LSD1 and IFITM3. HEK293T cells were transfected with HA-IFITM3 and FLAG-LSD1. Forty-eight hours later, cells were infected with WSN virus at MOI = 2. Cells were then collected at the indicated time-point post-infection and were immunoprecipitated with anti-HA antibodies plus protein A/G beads. HA-IFITM3 and FLAG-LSD1 were detected as above, and levels of viral NP protein and β-Actin were shown in whole cell lysates (Input). (B) HEK293T cells grown in six-well plates were transfected with 0.5μg HA-IFITM3 and 1μg FLAG-LSD1. Forty-eight hours later, cells were infected with VSV at MOI = 0.02. Cells were then collected at the indicated time-point post-infection and were immunoprecipitated with 1μg anti-IFITM3 antibody plus protein A/G beads. Protein blots were probed with the antibodies as indicated, data were repeated at least three times.